Immunohistochemical localization of sperm-preserving proteins in the ram reproductive tract.

Running head: Sperm preserving proteins Immunohistochemical Localization of Sperm Preserving Proteins in the Ram Reproductive Tract

Expression of cystatin C in the female reproductive tract and its effect on human sperm capacitation

BACKGROUND Cystatin C (CST3), a cysteine protease inhibitor in seminal plasma, is expressed in animal uteri. However, its expression in the human female reproductive tract and its effect on human sperm capacitation are unclear. METHODS The cellular localization of CST3 was observed using immunohistochemistry. The binding of CST3 to sperm was examined using immunocytochemistry. Sperm motility ...

Effect of different levels of egg yolk on ram sperm coating and preserving at 5°C

Experiment was conducted to evaluate the effect of different levels of egg yolk for coating and storing spermatozoa. Ejaculates were collected from four rams. In each session, second ejaculates (n=4) were collected in a tube containing 1 mL coating buffers which were prepared by 10, 15 or 20% egg yolk plus Tris-fructose. Samples were pooled, centrifuged and the supernatant removed in the labora...

Immunocytochemical localization and biochemical characterization of two seminal plasma proteins that protect ram spermatozoa against cold shock.

We have already shown that seminal plasma proteins in the ram can repair cold-shock sperm membrane damage and that the addition of seminal plasma proteins before cold-shock treatment prevents sperm membrane injury. In this study, we prove that 2 protein bands of approximately 14 (P14) and 20 (P20) kd isolated from seminal plasma are responsible for this protective effect. In vitro capacitation ...

Sperm Transport in the Mare Reproductive Tract: A Review

The objective of this study was to review the literature on sperm transport in the mare. During this process the sperm cells must reach the oviducts in order to fertilize the oocyte, but before reaching this site it must go through the uterus that is a hostile environment at this moment since there is an inflammatory reaction in this location that aims to eliminate excess spermatozoa and bacter...